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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Cancer Res. 2016 Feb 1;76(6):1463–1475. doi: 10.1158/0008-5472.CAN-15-2383

Figure 4. Cooperative regulation of Cyclin D1 by HER2 and SRC-3.

Figure 4

A) E2F1 ChIP-seq peaks were called from published resources and aligned to hg19 for parsing with HER2-regulated genes from the microarray. The pie graph indicates a majority of HER2-regulated genes contained at least one E2F1 binding site in the extended promoter region (EPR).

B) E2F1 and SRC-3 binding from published ChIP-seq at the enhancer 2 (enh2) region of CCND1 (striped bar).

C) E2F1 and SRC-3 binding by ChIP assay to the CCND1 enhancer region.

D) HER2 depletion decreases SRC-3 binding to the CCND1 enhancer.

E) Lapatinib treatment reduces CCND1 transcript levels and dismisses SRC-3 and H3K27ac from the CCND1 enhancer.