A) E2F1 ChIP-seq peaks were called from published resources and aligned to hg19 for parsing with HER2-regulated genes from the microarray. The pie graph indicates a majority of HER2-regulated genes contained at least one E2F1 binding site in the extended promoter region (EPR).
B) E2F1 and SRC-3 binding from published ChIP-seq at the enhancer 2 (enh2) region of CCND1 (striped bar).
C) E2F1 and SRC-3 binding by ChIP assay to the CCND1 enhancer region.
D) HER2 depletion decreases SRC-3 binding to the CCND1 enhancer.
E) Lapatinib treatment reduces CCND1 transcript levels and dismisses SRC-3 and H3K27ac from the CCND1 enhancer.