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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Cancer Res. 2016 Feb 1;76(6):1429–1440. doi: 10.1158/0008-5472.CAN-15-1115

Figure 3.

Figure 3

MCPIP1 selectively inhibits the mRNA expression of anti-apoptotic genes. (A) Clustergram of the apoptosis related genes affected differentially in MDA-MB-231/Tet-On cells at 32 hours after adding Dox (500 ng/ml). MCPIP1: cells with Dox; Control: cells without Dox. MDA-MB-231/Tet-On cells were induced to express MCPIP1 by Dox (500 ng/ml) for different times. Then total RNA was collected to detect mRNA levels of selected anti-apoptotic genes by qRT-PCR, including bcl2l1 (B), bric3 (C), bcl3 (D), and relb (E). The mRNA levels of pro-apoptotic genes in the above cells were measured by qRT-PCR, including bad (F), ripk2 (G), fas (H), and dedd2 (I). MDA-MB-231/Tet-On cells were induced to express GFP/MCPIP1 fusion protein by Dox (500 ng/ml) for 36 h, followed by lysate extraction, immunoprecipitation with anti-GFP antibody or IgG, and RNA extraction. RT-PCR was performed to detect anti-apoptotic gene transcripts (J) and pro-apoptotic gene transcripts (K). IL-6 transcript was used as positive control and GAPDH served as negative control.