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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Acta Biomater. 2015 Sep 16;34:93–103. doi: 10.1016/j.actbio.2015.09.019

Figure 5.

Figure 5

Demonstration of hMSC encapsulation in hydrogels formed using differential wettability patterning. All hydrogels presented CRGDS and were crosslinked with a MMP-degradable peptide. ad) Viability and density of hMSCs encapsulated in hydrogel spots presenting 2 mM CRGDS and formed using precursor solutions with varying concentrations of hMSC. a) Maximum intensity projection created by stacking images of the hydrogel acquired at 3 different focal planes in the hydrogel. c) Correlation of encapsulated hMSC density with hMSC concentration in precursor solution. e) Viability of ~500,000 hMSCs encapsulated in hydrogels spots containing varying concentrations of immobilized CRGDS peptide. Sample size: n ≥ 5 (b, d, e), n=3 (c). Statistical significance was determined by ANOVA followed by Tukey-Kramer test, whereby * denotes p<0.05 and “NS” denotes no statistical significance.