Table 1.
Primers and Probes for Semiquantitative Real-Time RT-PCR
| Gene | Oligo | Primer sequence |
|---|---|---|
| DNA polymerase (ORF9) | Forward | 5’-ACAGCAGCTGGCCATAAAGG-3’ |
| Reverse | 5’-TCCTGCCCTGGAAAGTGATG-3’ | |
| Probe | 5’-CCTCTGGAATGTTGCCTTGCCTCCA-3’ | |
| β-Actin | Forward | 5’-CCGTGAAAAGATGACCCAGATC-3’ |
| Reverse | 5’-CACAGCCTGGATGGCTACGT-3’ | |
| Probe | 5’-TTTGAGACCTTCAACACCCCCAGCCA-3’ | |
| Collagen I | Forward | 5’-TGACTGGAAGAGCGGAGAGTACT-3’ |
| Reverse | 5’-GGTCTGACCTGTCTCCATGTTG-3’ | |
| Probe | 5’-CTGCAACCTGGACGCCATCAAGG-3’ | |
| Collagen III | Forward | 5’-GGATCTGTCCTTTGCGATGAC-3’ |
| Reverse | 5GCTGTGGGCATATTGCACAA-3’ | |
| Probe | 5’-TGCCCCAACCCAGAGATCCCATTT-3’ | |
| Fibronectin | Forward | 5’-TCGAGCCCTGAGGATGGA-3’ |
| Reverse | 5’-GTGCAAGGCAACCACACTGA-3’ | |
| Probe | 5’-CTGCAGGGCCTCAGGCCGG-3’ | |
| IL23 p19 | Forward | 5’-CTCCCTACTAGGACTCAGCCAACT-3’ |
| Reverse | 5’-ACTCAGGCTGGGCATCTGTT-3’ | |
| Probe | 5’-AGCCAGAGGATCACCCCCGGG-3’ | |
| IL6 | Forward | 5’-GACTTCCATCCAGTTGCCTTCT-3’ |
| Reverse | 5’-CTGTTGGGAGTGGTATCCTCTGT-3’ | |
| Probe | 5’-TGACAACCACGGCCTTCCCTACTTCA-3’ | |
| IL1β | Forward | 5’-GAGCCCATCCTCTGTGACTCA-3’ |
| Reverse | 5’-GTTGTTCATCTCGGAGCCTGTAG-3’ | |
| Probe | 5’-AACCTGCTGGTGTGTGACGTTCCCA-3’ | |
| IL12 p35 | Forward | 5’-GTTGCCTGGCTACTAGAGAGACTTC-3’ |
| Reverse | 5’-GCACAGGGTCATCATCAAAGAC-3’ | |
| Probe | 5’-ACAACAAGAGGGAGCTGCCTGCC-3’ | |
| IFN-γ | Forward | 5’-GCAACAGCAAGGCGAGAAA-3’ |
| Reverse | 5’-GCTGGATTCCGGCAACAG-3’ | |
| Probe | 5’-AGGTCAACAACCCACAGGTCCAGCG-3’ |