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. 2016 Mar 17;7:96. doi: 10.3389/fimmu.2016.00096

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Copyright © 2016 Haileselassie, Navis, Vu, Qazi, Rethi and Sverremark-Ekström.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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L. reuteri-CFS upregulates CD14 and downregulates DC-SIGN expression on RA-DC. RA-DC and Mo-DC were treated with L. reuteri-CFS or LPS or for 24 h. Box plot graphs (A–E) show the percentage of CD11c⁺ cells expressing CD103 (A), CD1d (B), HLA-DR (C), CD83 (D) and CD86 (E) in RA-DCs and Mo-DC post-treatment. Representative heat map showing MFI fold changes of the surface markers HLA-DR, CD83, CD86, and DC-SIGN of CD11c⁺ RA-DC and Mo-DC post-treatment in comparison to culture medium is shown in (F). (G,H) shows the percentages of DC-SIGN⁺ (G) and CD14⁺ (H) CD11c⁺ cells in RA-DC and Mo-DC populations post-treatment. N ≥ 5, ***P < 0.001, **P < 0.01, and *P < 0.05.