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. 2016 Feb 10;49(1):21–28. doi: 10.1267/ahc.15022

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2016 The Japan Society of Histochemistry and Cytochemistry

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Immunohistochemical staining in KN-3 cells and Reverse-transcriptase PCR analysis for the expression of Hsp25 and VNUT in heat-treated KN-3 cells. (A) Immunofluorescence staining for VNUT and nestin and DAPI and merged in KN-3 cells. (B) Expression of Hsp25 was observed in KN-3 cells at 1, 3, and 6 hr after heat treatment. VNUT was most strongly expressed at 1 hr after heat treatment, subsequent to which the expression decreased gradually.