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. 2016 Feb 10;49(1):21–28. doi: 10.1267/ahc.15022

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2016 The Japan Society of Histochemistry and Cytochemistry

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — ATP release by KN-3 cells after heat stress. (A) The expression of VNUT in non-heat-treated or heat-stressed KN-3 cells after siRNA or non-siRNA KN-3 cells. The expression of VNUT was strongest in heat-stressed KN-3 cells after non-siRNA treatment. (B) ATP release by heat-stressed KN-3 cells was significantly greater than the control (not heat treated). The values represent means±S.E. ** p<0.05. (C) The effect of heat stress under siRNA on ATP release by KN-3 cells. The increased ATP release after heat stress was decreased significantly by VNUT siRNA interference. The values represent means±S.E. **p<0.05.