Fig. 3.

ALP activity and mineralized matrix deposition in H₂O₂-induced HBMSCs cultured with or without HAMSCs. (A) Mineralized matrix deposition was measured at 21 days by Alizarin red S. (B) Micrographs of mineralized matrix deposition were measured by Image-pro Plus analysis (IPP). (C) ALP activity was measured at 14 days using ALP assay kit. Double bar, 12.5 mm; ^##P < 0.01 versus the HBMSCs group; ^*P < 0.05 and ^**P < 0.01 in contrast to the group treated with H₂O₂ alone.