Fig. 1.
HDAC3 regulates the expression of CAGE. (A) The indicated cancer cells were transiently transfected with wild type HDAC3 or mutant HDAC3 (HDAC3S424A). At 48 h post transfection, cell lysates were subjected to Western blot analysis. Cell lysates from SNU387 or Malme3M cells were also subjected to Western blot analysis. (B) Cell lysates isolated from the indicated cancer cells were subjected to ChIP assays (left panel). The indicated cancer cells were transiently transfected with the indicated siRNA (10 nM) or construct (1 μg). At 48 h post transfection, cell lysates were isolated and subjected to ChIP assays (right panel). (C) The indicated cancer cells were transiently transfected with the indicated siRNA (10 nM) or construct (1 μg). At 48 h post transfection, cell lysates were subjected to Western blot analysis.