Fig. 5.

HDAC3 regulates the activation of EGR and induces an interaction between CAGE and EGFR. (A) Cell lysates isolated from the indicated cancer cells were subjected to Western blot analysis. (B) Malme3M cells were transiently transfected with the indicated siRNA (each at 10 nM). At 48 h after transfection, cell lysates were immunoprecipitated with the indicated antibody (2 μg/ml), followed by Western blot analysis. Cell lysates were also subjected to Western blot analysis. (C) Cell lysates isolated from the indicated cancer cells were immunoprecipitated with the indicated antibody (2 μg/ml), followed by Western blot analysis. (D) The indicated cancer cells were treated with various concentrations of gefitinib for 48 h, followed by MTT assays. (E) The indicated cancer cells were treated with gefitinib (1 μM) for 48 h, followed by Western blot analysis (lower panel).