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. 2015 Dec 16;39(3):250–257. doi: 10.14348/molcells.2016.2290

Fig. 2.

Fig. 2.

The Ubi:CaPUB1 transgenic rice plants displayed decreased tolerance to drought stress. (A) Light-grown, 6-week-old wild-type and T3 Ubi:CaPUB1 transgenic rice plants were subjected to drought stress (left panel). Survival rates of wild-type and CaPUB1-overexpressing transgenic lines were determined after drought stress. Data represent means ± SD (n = 38 plants) from four biological replicates for line #1 and five biological replicates for line #2 (right panel). (B) Total chlorophyll content of wild-type and CaPUB1-overexpressing plants before and after drought stress. Wild-type and Ubi:CaPUB1 transgenic plants were grown for 6 weeks and further grown with or without irrigation. Total amounts of chlorophyll a + chlorophyll b in leaf discs were measured. Data indicate means ± SD (n = 20 plants) from two independent experiments. (C) CaPUB1-overexpressing rice plants lost water more rapidly than wild-type plants during drought stress. Aerial parts of 5-week-old wild-type and CaPUB1-overexpressing T3 transgenic plants were detached and their fresh weights were measured at indicated time points. Data represent means ± SD from three independent experiments.