Fig. 4.

Real-time qRT-PCR analysis of cold stress-inducible genes in wild-type and Ubi:CaPUB1 transgenic plants. Total RNA was extracted from wild-type and two CaPUB1-overexpressing independent lines before and after cold treatment (4°C). Total RNA was analyzed by qRT-PCR using the gene-specific primer sets listed in Table 1. The graphs indicate the relative expression levels of OsDREB1A, OsDREB1B, OsDREB1C, and OsCytochromeP450 in wild-type and Ubi:CaPUB1 plants. The mean values of the three technical replicates were normalized to the level of OsACTIN mRNA as an internal control.