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. 2015 Dec 16;39(3):250–257. doi: 10.14348/molcells.2016.2290

Fig. 4.

Fig. 4.

Real-time qRT-PCR analysis of cold stress-inducible genes in wild-type and Ubi:CaPUB1 transgenic plants. Total RNA was extracted from wild-type and two CaPUB1-overexpressing independent lines before and after cold treatment (4°C). Total RNA was analyzed by qRT-PCR using the gene-specific primer sets listed in Table 1. The graphs indicate the relative expression levels of OsDREB1A, OsDREB1B, OsDREB1C, and OsCytochromeP450 in wild-type and Ubi:CaPUB1 plants. The mean values of the three technical replicates were normalized to the level of OsACTIN mRNA as an internal control.