Table 1. Proteins identified in HES from spots.
Tabulation of spot numbers and identifications
| Spot | Identity | Domain | Hpb adult database | Score | Peptides | Accession No |
|---|---|---|---|---|---|---|
| 1 | VAL-3.1 | Double SCP | isotig05790 | 64 | 2 | JF914909 |
| 2 | MEP-1 Zinc metalloprotease | Peptidase M13 | isotig02967 | 144 | 5 | (truncated) |
| 3 | CHI-1 Chitinase | Chitinase GH19 | isotig04840 | 268 | 3 | • • • • • • • • |
| 4 | VAL-3.1 | Double SCP | isotig05790 | 462 | 6 | JF914909 |
| 5 | VAL-2.1/2.2/2.3 (a) | Double SCP | isotig03106 | 43 | 1 | JF914906 |
| 6 | VAL-2.1/2.2/2.3 (a) | Double SCP | isotig03106 | 266 | 7 | JF914906 |
| 7 | VAL-2.1/2.2/2.3 (a) | Double SCP | isotig03106 | 206 | 6 | JF914906 |
| 8 | VAL-2.1/2.2/2.3 (a) | Double SCP | isotig03106 | 32 | 1 | JF914906 |
| 9 | VAL-2.1/2.2/2.3 (a) | Double SCP | isotig03106 | 429 | 7 | JF914906 |
| 10 | VAL-5 | Double SCP | isotig04839 | 186 | 8 | JF914911 |
| 11 | VAL-5 | Double SCP | isotig04839 | 146 | 10 | JF914911 |
| 12 | VAL-5 | Double SCP | isotig04839 | 132 | 8 | JF914911 |
| 13 | VAL-5 | Double SCP | isotig04839 | 145 | 5 | JF914911 |
| 14 | VAL-5 | Double SCP | isotig04839 | 169 | 7 | JF914911 |
| 15 | VAL-5 | Double SCP | isotig04839 | 121 | 4 | JF914911 |
| 16 | VAL-9 | Double SCP | isotig05765 | 495 | 10 | JF914917 |
| 17 | ACE-1 Acetylcholinesterase | Esterase lipase | isotig05694 | 21 | 1 | JF439067 |
| 18 | ACE-1 Acetylcholinesterase | Esterase lipase | isotig05694 | 32 | 2 | JF439067 |
| 19 | ACE-1 Acetylcholinesterase | Esterase lipase | isotig05694 | 53 | 2 | JF439067 |
| 20 | VAL-1.1 | Double SCP | isotig06320 | 16 | 1 | JF914902 |
| 21A | VAL-1.2 | Double SCP | isotig03069 | 43 | 1 | JF914903 |
| 21B | VAL-1.4 | Double SCP | isotig01653 | 38 | 1 | JF914905 |
| 22A | VAL-1.2 | Double SCP | isotig03069 | 129 | 4 | JF914903 |
| 22B | VAL-1.1 | Double SCP | isotig06320 | 103 | 4 | JF914902 |
| 22C | VAL-1.4 | Double SCP | isotig01653 | 70 | 2 | JF914905 |
| 23A | VAL-1.2 | Double SCP | isotig03069 | 419 | 5 | JF914903 |
| 23B | VAL-1.1 | Double SCP | isotig06320 | 402 | 5 | JF914902 |
| 24A | VAL-1.1 | Double SCP | isotig06320 | 613 | 6 | JF914902 |
| 24B | VAL-1.2 | Double SCP | isotig03069 | 385 | 5 | JF914903 |
| 25 | VAL-6 | Double SCP | isotig03505 | 401 | 4 | JF914912 |
| 26 | Enolase (b) | Enolase | isotig06965 | 103 | 3 | (truncated) |
| 27 | VAL-8.1 | Double SCP | isotig02308 | 257 | 6 | JF914916 |
| 28 | MEP-3 Zinc metalloprotease | Peptidase M13 | isotig05155 | 177 | 3 | (truncated) |
| 29 | APY-1.1 Apyrase | Apyrase | isotig02250 | 68 | 3 | JF721961 |
| 30 | APY-3 Apyrase | Apyrase | isotig03589 | 188 | 5 | JF721966 |
| 31 | APY-2 Apyrase | Apyrase | isotig07051 | 213 | 6 | JF721965 |
| 32 | APY-2 Apyrase | Apyrase | isotig07051 | 323 | 3 | JF721965 |
| 33 | APY-2 Apyrase | Apyrase | isotig07051 | 185 | 5 | JF721965 |
| 34 | PHP-1, PHA-domain protein | PHA02954 | isotig03547 | 180 | 4 | • • • • • • • • |
| 35 | VAL-4 | Single SCP | isotig10387 | 127 | 3 | JF914910 |
| 36A | LYS-1 Lysozyme | Muramidase GH25 | isotig08802 | 411 | 6 | • • • • • • • • |
| 36B | LYS-3 Lysozyme | Muramidase GH25 | isotig08606 | 126 | 3 | • • • • • • • • |
| 37A | LYS-1 Lysozyme | Muramidase GH25 | isotig08802 | 483 | 6 | • • • • • • • • |
| 37B | LYS-3 Lysozyme | Muramidase GH25 | isotig08606 | 170 | 3 | • • • • • • • • |
| 38A | VAL-7.3 | Single SCP | isotig02284 | 94 | 3 | JF914915 |
| 38B | VAL-7.2 | Single SCP | isotig01525 | 61 | 2 | JF914914 |
| 39 | VAL-7.2 | Single SCP | isotig01525 | 48 | 1 | JF914914 |
| 40 | VAL-7.1 | Single SCP | isotig01524 | 296 | 6 | JF914913 |
| 41 | NAS-1.1/1.2 Nematode Astacin (c) | ZnMc astacin-like | isotig04178 | 280 | 4 | (truncated) |
| 42 | NAS-3.3 Nematode Astacin (d) | ZnMc astacin-like | isotig01791 | 141 | 2 | (truncated) |
| 43 | Galectin | Double GLECT | isotig09082 | 219 | 4 | (truncated) |
| 44 | VAL-10 | Single SCP | isotig04979 | 27 | 1 | JF914918 |
| 45 | LYS-2 Lysozyme | Muramidase GH25 | isotig05074 | 220 | 4 | • • • • • • • • |
| 46 | LYS-2 Lysozyme | Muramidase GH25 | isotig05074 | 179 | 3 | • • • • • • • • |
| 47 | Vitellogenin | VWD | contig00471 | 64 | 2 | (truncated) |
| 48 | NSP-4 Novel Secreted Protein | None | isotig11873 | 60 | 1 | • • • • • • • • |
| 49 | NSP-16 Novel Secreted Protein | None | isotig05257 | 39 | 2 | • • • • • • • • |
| 50 | Myoglobin | Globin | isotig04274 | 64 | 1 | • • • • • • • • |
| 51 | Myoglobin | Globin | isotig04274 | 125 | 3 | • • • • • • • • |
| 52 | Myoglobin | Globin | isotig04274 | 94 | 3 | • • • • • • • • |
| 53 | TTR-1 Transthyretin-related | DUF290 | isotig04612 | 452 | 6 | • • • • • • • • |
Accession numbers from NCBI GenBank are given where full-length sequences are available.
(a)
Peptides are common to all three variants of VAL-2; isotig and Accession number given are for VAL-2.1. Isotig and accession numbers for VAL-2.2 and -2.3 are isotig07425/JF914907 and isotig03105/JF914908.
(b)
1 additional peptide matches isotig18996 coding for N-terminal segment of Enolase separated from isotig 06965 by gap of ~27 nt.
(c)
Peptides are common to NAS-1.1 and -1.2; latter is isotig04179; peptides common to both
(d)
Two additional peptide matches to each of variants NAS 3.1 (isotig01799) and NAS-3.4 (isotig 01793)