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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Cell Signal. 2016 Jan 7;28(4):294–306. doi: 10.1016/j.cellsig.2016.01.001

Fig 2.

Fig 2

Gravin-V5/His expression alters subcellular PKA activity. AN3 CA cells were co-transfected with gravin-V5/His and AKAR3 constructs containing targeting sequences for non-raft membrane (CAAX), membrane raft (Lck), or cytosolic (NES) localization. Representative fluorescent images show the distribution the AKAR3 constructs. Expression of gravin increased forskolin-stimulated PKA activity in both non-raft (A,B), and raft (C,D) targeted AKAR3 constructs. Conversely, gravin reduced PKA activity in the cytosol (E,F). Mann-Whitney Rank Sum tests revealed significant differences between treatments as indicated by asterisks (p < 0.05); error bars denote SD. Scale bar = 10 μm.