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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Cell Signal. 2016 Jan 7;28(4):294–306. doi: 10.1016/j.cellsig.2016.01.001

Fig. 7.

Fig. 7

Thapsigargin triggers gravin redistribution and abolishes gravin-mediated elevation in plasma membrane PKA activity. Representative fluorescent micrographs (A) illustrate the redistribution of gravin-EGFP away from the plasma membrane by thapsigargin. Gravin-V5/His redistribution was confirmed after FRET experiments by quantifying the percentage of cells showing gravin localization at the membrane, shown in the box plot represented in B (Mann-Whitney Rank Sum Test, p < 0.05). Graphs C,D show that gravin expression increased forskolin-stimulated PKA activity at the membrane, but this increase was not observed in cells expressing gravin and pretreated with the calcium-elevating agent thapsigargin (10 μm) for 30 min. Thapsigargin pretreatment had no effect on forskolin-stimulated PKA activity in the absence of gravin. One-way ANOVA on ranks followed by Kruskal-Wallis post hoc showed significant differences between treatments as indicated by asterisks. Error bars denote SD.