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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Metabolism. 2015 Dec 29;65(4):522–532. doi: 10.1016/j.metabol.2015.12.011

Figure 1. Schematic construction of the ERα-ZsGreen transgenic mouse allele.

Figure 1

The BAC clone 283O2 covers 241 kb around Esr1 genomic sequence (5’, 169 kb; 3’, 72 kb), which contains exon1–4 out of the Esr1 gene. For BAC recombination cassette, 393-bp of 5’ flanking region and 148-bp of 3’ flanking region were used as homology arms. By homologous recombination in bacteria, the ZsGreen cDNA was introduced into translation start site of the Esr1 gene in the BAC clone.