TABLE 1.

Experimental design for testing of nsp1β mutants in nursery pigs

Procedure	dpi	Pigs in treatment group
		Negative control (group 1)	WTe (group 2)	R128A (group 3)	R129A (group 4)	RR129AA (group 5)
Blood collectiona	0	1–9c	10–18	19–27	28–36	37–45
	1	1–9	10–18	19–27	28–36	37–45
	2	1–9	10–18	19–27	28–36	37–45
	5	1–9	10–18	19–27	28–36	37–45
	14	4–9	14–18	22–27	31–36	40–45
	28	7–9	17, 18	25–27	34–36	43–45
Animal terminationb	7	1–3d	10, 11, 13	19–21	28–30	37–39
	21	4–6	14–16	22–24	31–33	40–42
	35	7–9	17, 18	25–27	34–36	43–45

^aAt 0, 1, 2, 5, 14, and 28 dpi, plasma and PBMCs were collected.

^bAt 7, 21, and 35 dpi, 3 pigs from each group were terminated, and their plasma, PBMCs, bronchoalveolar lavage fluid, and lung tissue samples were collected.

^cPigs for blood collection at the indicated time point.

^dPigs terminated at the indicated time point.

^ePig 12 died before 7 dpi.