TABLE 2.

Sequence analysis of the nsp1β coding region in viruses recovered from infected pigs

Group	Pig	Designed mutation	Position of 2nd-site mutation, nucleotide substitution, amino acid substitutiona
14 dpi
vR129A	31	Reversion (GCG to AGG)
	32	Stable	122, UCU to CCU, Ser to Pro
	33	Stable	122, UCU to CCU, Ser to Pro
	34	Stable	122, UCU to CCU, Ser to Pro
	35	Stable	122, UCU to CCU, Ser to Pro
	36	Stable	122, UCU to CCU, Ser to Pro
21 dpi
WT	14		9, GAC to GGC, Asp to Gly
	15		9, GAC to GGC, Asp to Gly
	16		9, GAC to GGC, Asp to Gly
vR128A	22	Stable	9, GAC to GGC, Asp to Gly
			122, UCU to CCU, Ser to Pro
	23	Stable	9, GAC to GGC, Asp to Gly
			122, UCU to CCU, Ser to Pro
	24	Stable	9, GAC to GGC, Asp to Gly
			109, CAU to C(A/G)U, His to His/Arg
			122, UCU to CCU, Ser to Pro
			141, CUA to C(U/C)A, Leu to Leu/Pro
vR129A	31	Reversion (GCG to AGG)	9, GAC to GGC, Asp to Gly
	32	Stable	9, GAC to GGC, Asp to Gly
			87, GAA to GA(A/G)
			122, UCU to CCU, Ser to Pro
	33	Stable	9, GAC to GGC, Asp to Gly
			122, UCU to CCU, Ser to Pro
			169, UCU to (C/U)CU, Ser to Ser/Pro
vRR129AA	40	Stable
	41	Stable
	42	Stable
35 dpi
WT	17		9, GAC to GGC, Asp to Gly
			126, CUA to CU(A/G)
	18		9, GAC to GGC, Asp to Gly
vR128A	27	Stable	9, GAC to GGC, Asp to Gly
			109, CAU to CGU, His to Arg
			122, UCU to CCU, Ser to Pro
vR129A	34	Stable	9, GAC to GGC, Asp to Gly
			122, UCU to CCU, Ser to Pro
			132, UUU to (G/U)UU, Phe to Val
	34		141, CUA to CU(A/G)
			169, UCU to (C/U)CU, Ser to Ser/Pro
	35	Stable	9, GAC to GGC, Asp to Gly
			122, UCU to CCU, Ser to Pro
	36	Stable	9, GAC to GGC, Asp to Gly
			122, UCU to CCU, Ser to Pro
			169, UCU to CCU, Ser to Pro
vRR129AA	44	Stable	9, GAC to GGC, Asp to Gly
	45	Stable	9, GAC to GGC, Asp to Gly

^aNumbers refer to codon positions in the nsp1β coding region.