FIG 2.

miR-33a-5p downregulates the replication of JEV. (A to D) HEK293T cells were transfected with miR-33a-5p mimics for 24 h and then infected with JEV at an MOI of 1.0. The cells were harvested at 24 and 48 h postinfection, respectively. (A) According to the previous method, JEV NS5 protein levels were determined by Western blotting and normalized to GAPDH. (B) JEV mRNA levels were determined by quantitative real-time PCR and normalized to β-actin. (C) miR-33a-5p levels were determined by quantitative real-time PCR and normalized to U6. (D) JEV titers were determined by plaque assay in BHK cells. (E) HEK293T cells were transfected with different concentrations of miR-33a-5p mimics for 24 h and then infected with JEV at an MOI of 1.0. At 48 h postinfection, the cells were collected, and JEV NS5 protein levels were determined by Western blotting and normalized to GAPDH. JEV mRNA levels were determined by quantitative real-time PCR and normalized to β-actin. Data are shown as means ± the SEM of at least three independent experiments. *, P < 0.05; ***, P < 0.001.