FIG 8.

miR-33a-5p-mediated regulation of JEV replication is achieved through targeting EEF1A1. (A to E) HEK293T cells were cotransfected with miR-33a-5p mimics or control miRNA mimics and Myc-tagged EEF1A1 plasmid or vector for 24 h, and infected with JEV at an MOI of 1.0 or transfected with JEV replicon, then cells were harvested at 36 h postinfection or transfection. (A and B) According to the previous method, JEV NS5 and EEF1A1 protein levels were determined by Western blotting and normalized to GAPDH. (C and D) JEV mRNA levels were determined by quantitative real-time PCR and normalized to β-actin. (E) JEV titers were determined by plaque assay in BHK cells. Data are shown as means ± the SEM of at least three independent experiments. *, P < 0.05; ***, P < 0.001.