FIG 1.

CHD1 interacts with the influenza virus polymerase complex. (A) A549 cells were either mock infected (−) or infected (+) with the VIC strain (3 PFU/cell, 4 h); cell extracts were obtained and were immunoprecipitated (IP) using CHD-specific or control (IgG) antibodies. Proteins were monitored in Western blots probed with appropriate antibodies. Input, A549 cell extracts. (B) A549 cells were infected with influenza virus (3 PFU/cell, 4 h); cells were fixed and were processed for immunofluorescence using anti-CHD1, anti-PB2, and anti-NP antibodies. DAPI was used to stain nuclei. The colocalization panels show the signals common to the two antibodies, obtained with the colocalization mask. Representative images from one of three experiments are shown.