FIG 2.

CHD1 silencing does not affect cell viability or RNA polymerase activity. HEK293T and A549 cells were infected with lentiviruses expressing CHD1 silencers or a control lentivirus (shTT). (A) Western blot analysis of CHD1 proteins. (B) The viability of transduced HEK293T and A549 cells was determined by the MTT assay measuring cell metabolic activity. (C) Quantification of EU and BrU incorporation as well as RNAP II staining in 300 A549 cells infected with lentiviruses expressing CHD1 silencers or a control lentivirus (shTT). The relative intensity found in each analysis was compared with the intensity of uninfected control cells. Bars represent means ± standard errors of the means; no statistical differences were found.