TABLE 1.
Affinity of antibodies to cyclic and linear epitope 412-422a
| MAb | C-epitope I |
L-epitope I |
||||
|---|---|---|---|---|---|---|
| Ka (1/M · s) | Kd (1/s) | KD (M) | Ka (1/M · s) | Kd (1/s) | KD (M) | |
| C1 | 4.84 × 105 | 1.20 × 10−2 | 2.48 × 10−8 | NB | ||
| C2 | 4.07 × 105 | 1.23 × 10−2 | 3.03 × 10−8 | NB | ||
| C3 | 3.21 × 105 | 1.01 × 10−2 | 3.14 × 10−8 | NB | ||
| C4 | 2.82 × 105 | 1.25 × 10−2 | 4.42 × 10−8 | NB | ||
| C5 | 4.34 × 105 | 5.52 × 10−3 | 1.27 × 10−8 | NB | ||
| C6 | 3.59 × 105 | 3.22 × 10−4 | 8.97 × 10−10 | NB | ||
| C7 | 3.56 × 105 | 2.44 × 10−4 | 6.86 × 10−10 | NB | ||
| AP33 | 1.21 × 104 | 8.62 × 10−4 | 7.11 × 10−8 | 9.99 × 105 | 5.34 × 10−4 | 5.35 × 10−10 |
a
Association and dissociation rates and dissociation constants were obtained by SPR for the binding of C-epitope I and L-epitope to MAbs C1 to C7 and AP33. Data were analyzed using BIAevaluation (version 4.2) software. Ka, association constant; Kd, dissociation constant; NB, no binding.