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Viral FISH analysis of dual ATSV and Acidianus hospitalis fluorescence labeling by CARD-FISH. (A) A. hospitalis 16S rRNA-labeled cells. (B) ATSV-labeled cells. (C) Overlay of DAPI-stained cells (blue), A. hospitalis 16S rRNA-labeled cells (green), and ATSV-labeled cells (red). Examples of cells with colocalization of ATSV and A. hospitalis are indicated by red boxes.
