FIG 11.

The phosphorylation of IκB induced by Tax-1 is inhibited by CIITA. 293T cells were transfected with the empty pCDNA3 vector (pC) or with Tax-1 in the presence or absence of FLAG-tagged CIITA. Cells were treated with the proteasome inhibitor MG132 or with the vehicle DMSO. The expression of total IκB, P-IκB, and FLAG-tagged CIITA was assessed by Western blotting with the antibodies indicated. As a control for loading, the expression of α-tubulin (a tub) was also evaluated. The values to the left of the gels are molecular sizes in kilodaltons.