FIG 5.

CIITA expressed in T and monocytic cells inhibits Tax-1-mediated NF-κB activation. (A) Jurkat, Jurkat-GFPfCIITA, U937 cl10, U937 cl34, and U937 cl10-fCIITA cells were transfected with pNF-κB-Luc and phRL-CMV in the absence (black columns) or in the presence of proviral plasmid pACH (white columns). At 48 h posttransfection, the luciferase activity of the cell lysates was measured. (B and C) U937 clone 10 and 34 cells, respectively, were transfected with plasmid pACH. At 48 h posttransfection the cells were fixed; stained with anti-RelA, anti-Tax-1, and anti-CIITA antibodies; and analyzed by confocal microscopy as described in Materials and Methods.