FIG 6.

Effect of EqCXCL16 expression on EAV infection of stable HEK-EqCXCL16 cells. (A) Naive HEK-293T cells (row a) or stable HEK-EqCXCL16 cells expressing the EqCXCL16 protein (row b) were infected with EAV sVBSmCherry at an MOI of 1.0, and at 12 hpi, the cells were fixed with 4% PFA. Cells were analyzed by inverted immunofluorescence microscopy for mCherry expression. The same fields of cells were also analyzed by phase-contrast microcopy. Compared to the level of mCherry expression in naive HEK-293T cells, a significant increase in the level of mCherry expression was found in the stable HEK-EqCXCL16 cells (middle column). (B) The intensity of mCherry expression was quantitated using Nikon NIS-Elements AR (version 4.13.00) software. The data represent the means ± standard deviations from 3 independent experiments, and data were considered significant at a P value of <0.001 by Student's t test (***).