FIG 8.

Effect of polyclonal antibody-mediated blocking of EqCXCL16 on EAV infection. Stable HEK-EqCXCL16 cells were treated with either DMEM (a), 2-fold serial dilutions (from 1:10 to 1:160) of Gp anti-EqCXCL16 pAb (b to f), or prebleed guinea pig sera (g to k) for 2 h at 37°C. Cells were infected with EAV sVBSmCherry at an MOI of 1.0 in the presence of Gp anti-EqCXCL16 pAb or prebleed guinea pig sera. At 12 hpi, cells were fixed and analyzed by inverted fluorescence microscopy for the evaluation of mCherry expression. In the absence of antibody, stable cells infected with EAV sVBSmCherry expressed mCherry (a), whereas antibody treatment inhibited mCherry expression (b to e). The prebleed sera, however, did not inhibit mCherry expression (g to k).