FIG 1.

Schematic representation of dual-luciferase/dual-fluorescent reporter constructs. (A) The core pLuc(Reni)-AsLuc(Fire) plasmid contains genes for Renilla luciferase and firefly luciferase that are translated from the sense and antisense transcripts, respectively, as indicated. Variations in the DNA regions flanking the core are indicated. pA denotes the SV40 late polyadenylation signal. A polyadenylation signal for the sense transcript is located in the 3′ LTR. (B) Schematic representation of the bidirectional pHTLV-DsRed-AsEGFP-HTLV reporter construct.