FIG 3.

Tax activates sense transcription without affecting the level of antisense transcription. Luciferase activities corresponding to sense and antisense transcription are represented with solid and hatched bars, respectively. (A, C, and E) The graphs show relative luciferase activities corresponding to activation of 5′ LTR-controlled sense transcription by Tax from constructs containing the 3′ LTR (LTR/LTR) or the CMV promoter in place of the 3′ LTR (LTR/CMV) or with a with a deletion of the 3′ LTR (LTR/−) in HEK293T, CEM, or Jurkat cells as indicated. Fold activation denotes the average Tax activation over basal transcription levels. (B, D, and F) The graphs show relative luciferase activities corresponding to 3′ LTR-controlled antisense transcription in the absence or presence of Tax from constructs containing the 5′ LTR (LTR/LTR) or the CMV promoter in place of the 5′ LTR (CMV/LTR) or with a deletion of the 5′ LTR (−/LTR) in HEK293T, CEM, or Jurkat cells as indicated. All graphs display mean luciferase activities relative to the β-galactosidase internal control with values normalized to luciferase activities from the reporter construct with a deletion of both LTRs (−/−; set to 1). Data from HEK293T, CEM, and Jurkat cells are from six, four, and three independent experiments, respectively. Error bars denote standard deviations. Significant results as determined by Student's t test are indicated (*, P < 0.01; **, P < 0.001).