FIG 4.

(A) Neutralization susceptibility of autologous Envs to contemporaneous G37080 BCN plasma and its follow-up sample from the same donor. Neutralization titers (median ID₅₀) were obtained by titrating pseudotyped viruses expressing autologous Envs obtained from visit 1 and follow-up G37080 plasma to contemporaneous plasma antibodies. Note that both of the Envs obtained from follow-up G37080 plasma (visit 2) were found to be resistant to contemporaneous autologous plasma, while Envs obtained from visit 1 G37080 plasma were found to be sensitive to follow-up plasma antibodies. (B) Alignment of V1V2 amino acid sequences of sensitive and resistant autologous Envs obtained at both visits was done by using seqpublish, available at the HIV Los Alamos database (www.hiv.lanl.gov). Key residues that mediate autologous neutralization resistance are highlighted.