TABLE 4.
Dissection of specificity for autologous neutralization resistance
| Chimera and point mutant | Neutralization potency (ID50) |
|
|---|---|---|
| Fold changea | Effectb | |
| PG80v1.eJ7 Env backbone | ||
| V1V2 loop | ||
| PG80v2.eJ38 (V1V2) in v1.eJ7 | 45.75 | Decrease |
| PG80v2.eJ38 (V1) in v1.eJ7 | 27.97 | Decrease |
| PG80v2.eJ38 (V2) in v1.eJ7 | 1.35 | No effect |
| Point mutations | ||
| PG80v1.eJ7 (D133N) | 3.25 | Decrease |
| PG80v1.eJ7 (S143G) | 0.87 | No effect |
| PG80v1.eJ7 (D133N + S143G) | 2.88 | Decrease |
| PG80v1.eJ7 (T147P) | 8.21 | Decrease |
| PG80v1.eJ19 Env backbone | ||
| V1V2 loop | ||
| PG80v2.eJ38(V1V2) in v1.eJ19 | 23.05 | Decrease |
| PG80v2.eJ38 (V1) in v1.eJ19 | 28.61 | Decrease |
| PG80v2.eJ38 (V2) in v1.eJ19 | 1.87 | Increase |
| Point mutations | ||
| PG80v1.eJ19 (D133N) | 2.51 | Decrease |
| PG80v1.eJ19 (T139A) | 0.99 | No effect |
| PG80v1.eJ19 (T139A + T140D) | 2.64 | Increase |
| PG80v1.eJ19 (N143G) | 1.38 | No effect |
| PG80v1.eJ19 (T139A + T140D + N143G) | 2.16 | Increase |
| PG80v1.eJ19 (T145P) | 3.24 | Decrease |
| PG80v2.eJ38 Env backbone | ||
| V1V2 loop | ||
| PG80v1.eJ7 (V1V2) in v2.eJ38 | 26.56 | Increase |
| PG80v1.eJ7 (V1) in v2.eJ38 | 49.62 | Increase |
| PG80v1.eJ7 (V2) in v2.eJ38 | 1.07 | No effect |
| PG80v1.eJ19(V1V2) in v2.eJ38 | 12.81 | Increase |
| PG80v1.eJ19 (V1) in v2.eJ38 | 37.60 | Increase |
| PG80v1.eJ19 (V2) in v2.eJ38 | 0.94 | No effect |
| Other regions in gp120 | ||
| PG80v1.eJ7 (V3C3) in v2.eJ38 | 0.84 | No effect |
| PG80v1.eJ7 (V3C3V4C4) in v2.eJ38 | 0.90 | No effect |
| PG80v1.eJ7 (C4V5C5) in v2.eJ38 | 1.08 | No effect |
| PG80v1.eJ19 (V3C3) in PG80v2.eJ38 | 1.09 | No effect |
| PG80v1.eJ19 (V3C3V4C4) in PG80v2.eJ38 | 1.15 | No effect |
| PG80v1.eJ19 (C4V5C5) in PG80v2.eJ38 | 0.96 | No effect |
| Point mutations | ||
| PG80v1.eJ19 V1 (T139A+T140D) in v2.eJ38 | 53.91 | Increase |
| PG80v2.eJ38 (N133D) | 4.72 | Increase |
| PG80v2.eJ38 (G143S) | 0.94 | No effect |
| PG80v2.eJ38 (N133D+G143S) | 3.63 | Increase |
| PG80v2.eJ38 (P147T) | 4.11 | Increase |
| Heterologous Env chimera | ||
| V1V2 loop | ||
| 16055-2.3 (25711-2.4 V1V2) | 18.38 | Increase |
| 25711-2.4 (16055-2.3 V1V2) | 2.03 | Decrease |
| CAP45 (25711-2.4 V1V2) | 16.84 | Increase |
| 25711-2.4 (CAP45-V1V2) | 10.54 | Decrease |
a
Fold changes in reciprocal dilution of plasma mediating 50% virus neutralization (ID50).
b
Fold increase or decrease in neutralization titer (ID50 values).