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. 2016 Feb 3;88(6):3235–3242. doi: 10.1021/acs.analchem.5b04649

Figure 6.

Figure 6

Multispot deposition of goat anti-IgG using recirculation with the MFP. (a) Image of the deposition of anti-IgG solution using 1.6 μL with concentrations of 5, 10, and 25 μg/mL and incubation times of 10 s, 1 min, 4 min, and 10 min. (b) Fluorescence intensity of zones shown in part a. (c) Photograph of 130 spots deposited with 1 μL of solution (170 spots in total). The flow direction was switched every ten spots. (d) Average fluorescence intensity measured on each spot compared with the predictions of our models. (e and f) Intrafootprint variation within one spot (<6% variation).