Table 1. Enzyme kinetics parameters of wild and recombinant SGT.

Variants	Km(mM)	BAPNAakcat (s−1)	kcat/Km(s−1·M−1)
BT	8.28 ± 0.33 × 10−1	5.03 ± 0.18 × 101	6.08 ± 0.25 × 104
SGT	7.88 ± 0.16 × 10−2	1.21 ± 0.42 × 103	1.53 ± 0.05 × 107
Exmt	3.05 ± 0.12 × 10−2	1.90 ± 0.10 × 103	6.23 ± 0.32 × 107
R145D	5.08 ± 0.30 × 10−2	3.58 ± 0.15 × 101	7.07 ± 0.30 × 105
R145I	3.42 ± 0.14 × 10−2	2.48 ± 0.10 × 103	7.25 ± 0.43 × 107
R145L	3.57 ± 0.18 × 10−2	1.35 ± 0.58 × 102	3.80 ± 0.17 × 106
R145M	4.14 ± 0.12 × 10−2	2.22 ± 0.08 × 102	5.35 ± 0.17 × 106
R145T	7.85 ± 0.31 × 10−2	2.47 ± 0.10 × 102	3.15 ± 0.13 × 106
R145V	9.20 ± 0.28 × 10−2	1.72 ± 0.07 × 101	1.87 ± 0.10 × 106

^aThe Lineweaver-Burk plots of substrate BAPNA was showed in Supplementary Fig. S8.