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. 2016 Mar 17;6:23317. doi: 10.1038/srep23317

Figure 3. p300 interacts with PPARα and promotes its activation and ketogenesis.

Figure 3

(A) Immunoblot showing recovery of PPARα from IPs of p300 prepared from HepG2 transfected with HA-tagged p300, HA-tagged p300 S89A, HA-tagged p300 LXXAA and PPARα expression vectors as indicated. (B–D) Effect of fasting on hepatic Aox-PPRE-luciferase reporter activity (B), serum total ketone body (C) as well as mRNA amounts for hepatic ketogenic genes (D) in mice injected with Ad-GFP or Ad-p300. Quantification of luminescent intensity of (B) was shown. (E–G) Effect of fasting on hepatic Aox-PPRE-luciferase reporter activity (E), serum total ketone body (F) as well as mRNA amounts for hepatic ketogenic genes (G) in mice injected with Ad-USi or Ad-p300i. Quantification of luminescent intensity of (E) was shown.