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. 2016 Mar 17;6:23317. doi: 10.1038/srep23317

Figure 4. SIK2 regulates PPARα activation and ketogenesis through p300 phosphorylation.

Figure 4

(A) Immunoblot showing p300 Ser89 phosphorylation and recovery of PPARα from IPs of p300 prepared from liver under ad lib, fasted or refed conditions as indicated. (B) Immunoblot showing p300 Ser89 phosphorylation and recovery of PPARα from IPs of p300 prepared from liver infected with Ad-GFP, Ad-SIK2 or Ad-USi, Ad-SIK2i as indicated. (C–E) Effect of fasting on hepatic Aox-PPRE-luciferase reporter activity (C), serum total ketone body (D) as well as mRNA amounts for hepatic ketogenic genes (E) in mice injected with Ad-GFP, Ad-SIK2 together with Ad-p300 or Ad-p300 S89A as indicated. Quantification of luminescent intensity of (C) was shown. (F) Immunoblot showing recovery of PPARα from IPs of p300 prepared from liver from (C). (G) Schematic of proposed mechanism in hepatocytes.