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. 2016 Jan 4;6(3):179–189. doi: 10.1002/2211-5463.12000

Figure 6.

Figure 6

PTP inhibition resulted in enhanced glucose uptake accompanied by increased insulin receptor phosphorylation. (A‐B) Differentiated C2C12 cells were treated with sodium stibogluconate (A), BMOV (B) and transfected with SHP‐1 siRNA (C) followed by measurement of insulin‐induced glucose uptake. Expression of mRNA was analysed to confirm efficient SHP‐1 downregulation and to rule out compensatory regulation of the insulin receptor and other PTPs (D). (*P < 0.05; **P < 0.01). (E) Dephosphorylation assay was performed with precipitated insulin receptor, which served as substrate for recombinant SHP‐1 and PTP1B. To visualize the dephosphorylation of the insulin receptor immunoblotting was done with depicted antibodies.