Table 3.
Antibodies used in the present study
| Primary antibody | Reference | Source | IHC dilution | WB dilution |
|---|---|---|---|---|
| DPL – purified boDpl67–81 | Gift of Dr Paltrinieri (Rondena et al. 28) | Rabbit | 1 : 150 | |
| PRP – Sha31 | (Feraudet et al. 36) | Mouse | 1 : 1000 | |
| Biotinylated PRP – b Sha31 | (Feraudet et al. 36) | Mouse | 1 : 50 000 | |
| Murine SHO | Gift of Dr Westaway (Watts et al. 15) | Rabbit | 1 : 500 | |
| Ovine SHO | Gift of Dr Peelman (Lampo et al. 37) | Rabbit | 1 : 50 | |
| Cyp17A | Gift of Dr Hales (Hales et al.38) | Rabbit | 1 : 200 |
| Secondary antibody | Reference | Conjugate | IHC dilution | WB dilution |
|---|---|---|---|---|
| Anti‐rabbit IgG‐Dylight 488 | 072‐03‐15‐06, KPL | Fluorescein | 1 : 200 | |
| Anti‐rabbit IgG‐Dylight 594 | 072‐09‐15‐06, KPL | Fluorescein | 1 : 200 | |
| Anti‐mouse IgG‐Cy3 | AP160C, Millipore | Cyanine 3 | 1 : 200 | |
| Anti‐rabbit IgG‐B | BA‐1000, Vector | Biotin | 1 : 200 | |
| Anti‐mouse‐IgG‐HRP strep |
Horseradish peroxydase Streptavidin |
1/20 000 | ||
| Fluorescein Streptavidin | SA‐5001, Vector | 1 : 200 | ||
| Texas Red® Streptavidin | SA‐5006, Vector | 1 : 200 | ||
| Mounting medium | Reference | |||
| Vectashield for fluorescence with DAPI | H‐1200, Vector |