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. 2015 Dec 29;6(1):50–55. doi: 10.1002/2211-5463.12005

Figure 1.

Figure 1

Specificity analysis of VASA promoter in vitro. (A) Construction of the VASA‐tdTOMATO vector. The 4.3 kb VASA promoter fragment was cloned into the vector of tdTOMATO. (B) Analysis of the expression of tdTOMATO in 293T, PK, PEF and MLTC‐1 cell lines. The CMV‐tdTOMATO vector was used as the positive control. (C) Construction of VASA‐Cre expression vector. The expression of Cre was controlled by the 4.3‐kb fragment of the pig VASA 5′‐flanking region, which was used to perform the SCNT in pig.