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. 2015 Dec 29;6(1):50–55. doi: 10.1002/2211-5463.12005

Figure 2.

Figure 2

Generation and identification of VASA‐Cre Tg pigs. (A, B) Two of four surviving founder Landrace (ID nos 2723 and 2725) (A) and two surviving founder mini‐pigs (ID nos 2735 and 2737) (B). (C, D) PCR identification of the Cre gene in Tg Landrace (C) and mini‐pigs (D). All of the Tg pigs except No. 2727 were positive. VASA‐Cre vector served as postive control and GAPDH was used as the internal control in PCR analysis; a wild‐type piglet genomic sample (WT) and distilled water served as negative control in PCR analysis.