Table 5.

Identified hemicellulose/pectin-degradation enzymes and spectrum counts in different carbon sources by wild-type 2HH and mutant S1M29 of Penicillium echinulatum at 96 h during submerged cultivation

Accession number	Identified proteins	CAZyme	Organism	MW (kDa)	Secretionb	Spectrum counta
						2HH				S1M29
						Glu	Gly	SCB	Cel	Glu	Gly	SCB	Cel
g1097	α-l-arabinofuranosidase	GH62 (CBM1)	Penicillium oxalicum	41	Y	0	0	0	0	0	0	1	3
g7308	α-l-arabinofuranosidase	GH43	Penicillium oxalicum	34	Y	0	2	2	0	0	0	1	0
g1098	α-l-arabinofuranosidase	GH43 (CBM1)	Penicillium oxalicum	62	Y	0	0	0	1	0	0	0	1

^aSecretomic analysis based on spectral counting. A quantitative analysis was conducted for samples grown on cellulose or SCB (mean of triplicates), while a semiquantitative analysis for samples grown on glucose or glycerol (one replicate) was performed. The complete protein reports are given in Additional file 3

^bThe secretion of each protein was verified by the softwares SignalP, SecretomeP and YLoc. When at least two of three softwares give a positive result the protein was considered secreted