Table 1.
Calculated dissociation constants for HIV-2 NC, MA, and GagΔp6 complexes with selected RNA
NaCl (mM) | Kd (nM) | ||
---|---|---|---|
NC | MA | GagΔp6 | |
TARpA RNA | |||
50 | 207 ± 9 | 431 ± 88 | 49 ± 5 |
100 | 285 ± 21 | 417 ± 74 | 52 ± 5 |
150 | 385 ± 16 | 430 ± 46 | 53 ± 5 |
200 | 541 ± 6 | 538 ± 68 | 65 ± 4 |
250 | 679 ± 16 | 593 ± 76 | 97 ± 7 |
500 | 1432 ± 22 | 2377 ± 209 | 965 ± 83 |
PBS RNA | |||
50 | 36 ± 3 | 335 ± 28 | 31 ± 1 |
100 | 117 ± 6 | 289 ± 4 | 31 ± 3 |
150 | 196 ± 9 | 290 ± 5 | 30 ± 2 |
200 | 277 ± 26 | 340 ± 25 | 31 ± 2 |
250 | 392 ± 37 | 382 ± 25 | 41 ± 3 |
500 | 2036 ± 212 | 2072 ± 98 | 314 ± 29 |
Ψ RNA | |||
50 | 7 ± 2 | 413 ± 52 | 27 ± 1 |
100 | 25 ± 1 | 300 ± 41 | 17 ± 1 |
150 | 71 ± 1 | 312 ± 29 | 15 ± 1 |
200 | 135 ± 4 | 357 ± 21 | 24 ± 2 |
250 | 240 ± 10 | 374 ± 19 | 26 ± 3 |
500 | 979 ± 26 | 1514 ± 210 | 125 ± 5 |