Table 5.

Genes validated between qRT-PCR and RNAseq methodologies, including fold change (FC), P-values and correlation coefficients (R)

	RNAseq		RT-qPCR		Correlation
Gene	FC	P-Value	FC	P-Value	R	P-Value
Period 1
SOCS3	−1.17	0.244	−0.61	0.0924	0.59	0.052
JAK2	1.15	0.227	0.71	0.602	0.61	0.044
STAT5B	−1.07	0.576	−0.69	1	0.57	0.031
IGF1	−2.53	<.0001	−1.06	0.001	0.48	0.013
IGFBP1	3.62	<.0001	3.91	<.0001	0.95	<0.001
IGFBP2	29.25	<.0001	8.45	<.0001	0.52	0.008
IGFBP3	−1.11	0.139	−0.84	0.684	0.63	0.045
IGFBP4	1.01	0.952	0.65	0.872	0.51	0.094
IGFBP5	−1.48	0.401	−1.13	0.999	0.70	0.012
IGFBP6	1.83	<.0001	1.74	<.0001	0.65	0.023
ALS	1.05	0.641	1.02	0.648	0.82	0.037
GHR	−1.89	<.0001	−1.39	0.0274	0.77	0.048
Period 2
SOCS3	1.05	0.722	0.065	0.963	0.49	0.089
JAK2	1.13	0.339	0.75	0.641	0.63	0.054
STAT5B	1.09	0.466	0.62	0.999	0.48	0.036
IGF1	−1.01	0.914	−0.76	0.995	0.72	0.037
IGFBP1	1.29	0.032	1.00	0.043	0.81	0.002
IGFBP2	1.04	0.755	0.51	0.910	0.59	0.054
IGFBP3	1.05	0.669	0.58	0.979	0.29	0.068
IGFBP4	−1.04	0.731	−0.83	0.993	0.42	0.047
IGFBP5	1.33	0.051	1.00	0.495	0.16	0.054
IGFBP6	−1.23	0.625	−0.77	0.990	0.57	0.024
ALS	−1.01	0.955	−0.88	1	0.49	0.078
GHR	1.03	0.048	1.09	0.041	0.86	0.025