Fig. 2.

Direct interaction of Pips to PfPP1. The binding capacity of recombinant Pips (25pmol/well) to biotin-PfPP1 (4pmol/well) was measured using an ELISA-based assay. a represents the binding of recombinant Pips identified by PP1-affinity column (gray bars). PfI2 (PF3D7_0320000) and PfI3 (PF3D7_1031700) were used as positive controls (white bars) and BSA was used as negative control (black bars). b represents the binding of Pips identified by Y2H screening (gray bars). AAN59960 and AAN59961 are human H2A and H2B proteins respectively which present high identity with PfH2A (PF3D7_0617800) and PfH2B (PF3D7_1105100). c represents the Pips identified by genome in silico analysis (gray bars). d contribution of the RVxF motifs to the binding of PF3D7_0220000 and PF3D7_0919900 to PfPP1 (gray bars). The interaction of PfPP1 to wild proteins was considered at 100 % binding. Results are presented as mean ± SD of 2 independent experiments preformed in duplicate. *p < 0.05, **p < 0.01 and ***p < 0.001 (Mann–Whitney U test), compared to the negative control