Table 2.
Pips identified by Yeast two-hybrid screening
| PlasmoDB Accession numbera | Namea | Growth on selective plates (number of isolated clones)b | RVxF motifsc | |
|---|---|---|---|---|
| In frame genes | PF3D7_0107600 | serine/threonine protein kinase, putative | TDO/A (1) | |
| PF3D7_0220000 | liver stage antigen 3 (LSA3) | QDO/A (2) | KKVRF | |
| PF3D7_0418300 | conserved Plasmodium protein, unknown function | QDO/A (1) | ||
| PF3D7_0520800 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0520900 | S-adenosyl-L-homocysteine hydrolase (SAHH) | TDO/A (1) | ||
| PF3D7_0610100 | Pre-mRNA-splicing factor SLU7, putative (SLU7) | TDO/A (1) | ||
| PF3D7_0611800 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0613800 | transcription factor with AP2 domain(s) (ApiAP2) | QDO/A (1) | RGVYF | |
| TDO/A (1) | ||||
| PF3D7_0623100 | nuclear polyadenylated RNA-binding protein NAB2, putative | TDO/A (2) | ||
| PF3D7_0718100 | exported serine/threonine protein kinase (EST) | TDO/A (1) | ||
| PF3D7_0720700 | phosphoinositide-binding protein, putative | TDO/A (1) | KIKF | |
| PF3D7_0724600 | protein kinase, putative | TDO/A (1) | ||
| PF3D7_0803400 | DNA repair protein rad54, putative | QDO/A (1) | ||
| PF3D7_0816600 | ClpB protein, putative (ClpB1) | TDO/A (1) | ||
| PF3D7_0917900 | heat shock protein 70 (HSP70-2) | TDO/A (1) | ||
| PF3D7_0919900 | regulator of chromosome condensation, putative | QDO/A (8) | KSVSF | |
| PF3D7_1008000 | histone deacetylase 2 (HDA2) | TDO/A (1) | ||
| PF3D7_1008100 | conserved Plasmodium protein, unknown function | TDO/A (1) | KSVSF | |
| PF3D7_1020900 | ADP-ribosylation factor (ARF1) | TDO/A (1) | ||
| PF3D7_1031600 | conserved Plasmodium protein, unknown function (GEXP15) | QDO/A (3) | KKVQF | |
| PF3D7_1105100 | histone H2B (H2B) | TDO/A (1) | ||
| PF3D7_1130700 | structural maintenance of chromosome protein, putative | TDO/A (2) | ||
| PF3D7_1202600 | conserved protein, unknown function | QDO/A (10) | KNVTF/KCVSF/KQVTF/RTVSF/KKVTF/KSVSF/KKVTF | |
| PF3D7_1205500 | zinc finger protein, putative | TDO/A (1) | ||
| PF3D7_1228600 | merozoite surface protein 9 (MSP9) | TDO/A (1) | ||
| PF3D7_1229600 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1303800 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1317600 | conserved Plasmodium protein, unknown function | TDO/A (1) | KYIYF | |
| PF3D7_1325800 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1346100 | protein transport protein SEC61 subunit alpha (SEC61) | TDO/A (1) | KGIEF | |
| PF3D7_1358200 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| Out of frame genes | mal_mito_2 | cytochrome c oxidase subunit 1 (COX1) | TDO/A (2) | |
| PF3D7_0110200 | FAD-linked sulfhydryl oxidase ERV1, putative (ERV1) | TDO/A (1) | KINF | |
| PF3D7_0202400 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0205700.1 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0207100 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0207500 | serine repeat antigen 6 (SERA6) | TDO/A (1) | ||
| PF3D7_0307700 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0310400 | parasite-infected erythrocyte surface protein (PIESP1) | TDO/A (1) | ||
| PF3D7_0312800 | 60S ribosomal protein L26, putative | TDO/A (1) | ||
| PF3D7_0317300 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0319500 | RNA binding protein, putative | TDO/A (1) | ||
| PF3D7_0407700 | conserved Plasmodium protein, unknown function | TDO/A (1) | RDIIF | |
| PF3D7_0417400 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0418000 | conserved Plasmodium protein, unknown function | TDO/A (2) | ||
| PF3D7_0418300 | conserved Plasmodium protein, unknown function | TDO/A (2) | ||
| PF3D7_0419900 | phosphatidylinositol 4-kinase, putative | QDO/A (2) | ||
| TDO/A (1) | ||||
| PF3D7_0420000 | zinc finger protein, putative | TDO/A (1) | ||
| PF3D7_0509200 | leucine-rich repeat protein (LRR2) | QDO/A (1) | ||
| PF3D7_0516600 | translation initiation factor IF-2 | TDO/A (1) | ||
| PF3D7_0516900 | 60S ribosomal protein L2 (RPL2) | TDO/A (1) | KVIF | |
| PF3D7_0520800 | conserved Plasmodium protein, unknown function | QDO/A (1) | ||
| PF3D7_0520900 | S-adenosyl-L-homocysteine hydrolase (SAHH) | TDO/A (1) | ||
| PF3D7_0525200 | conserved Plasmodium protein, unknown function | TDO/A (2) | KIEF/KDVLF | |
| PF3D7_0530000 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0532100 | early transcribed membrane protein 5 (ETRAMP5) | TDO/A (2) | ||
| PF3D7_0602000 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0604500 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0611700 | 60S ribosomal protein L39 (RPL39) | TDO/A (2) | ||
| PF3D7_0613800 | transcription factor with AP2 domain(s) (ApiAP2) | TDO/A (1) | ||
| PF3D7_0617200 | conserved Plasmodium protein, unknown function | QDO/A (1) | KQIGF | |
| PF3D7_0617800 | histone H2A (H2A) | QDO/A (2) | ||
| TDO/A (6) | ||||
| PF3D7_0625300 | DNA polymerase 1, putative | TDO/A (1) | ||
| PF3D7_0709300 | Cg2 protein (CG2) | TDO/A (1) | KVNF | |
| PF3D7_0719900 | conserved Plasmodium membrane protein, unknown function | TDO/A (1) | ||
| PF3D7_0721100 | conserved Plasmodium protein, unknown function | QDO/A (1) | ||
| TDO/A (1) | ||||
| PF3D7_0725300 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0806900 | conserved Plasmodium protein, unknown function | QDO/A (1) | KNIGF/KIRW | |
| PF3D7_0809400 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0811400 | conserved protein, unknown function | TDO/A (1) | ||
| PF3D7_0813200 | CS domain protein, putative | TDO/A (1) | ||
| PF3D7_0813300 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0814000 | 60S ribosomal protein L13-2, putative | QDO/A (2) | RVNF | |
| PF3D7_0815800 | vacuolar sorting protein VPS9, putative | TDO/A (1) | ||
| PF3D7_0818000 | conserved protein, unknown function | TDO/A (1) | ||
| PF3D7_0818900d | heat shock protein 70 (HSP70) | TDO/A (1) | ||
| PF3D7_0831700 | heat shock protein 70, putative (HSP70-x) | QDO/A (1) | ||
| PF3D7_0904000 | GTPase-activating protein, putative | TDO/A (1) | ||
| PF3D7_0919900 | regulator of chromosome condensation, putative | TDO/A (1) | ||
| PF3D7_0921700 | conserved Plasmodium protein, unknown function | QDO/A (1) | ||
| PF3D7_0924100 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_0932100 | protein MAM3, putative | TDO/A (1) | ||
| PF3D7_0933300 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1020200 | conserved Plasmodium protein, unknown function | TDO/A (1) | KNVFF | |
| PF3D7_1023400 | HORMA domain protein, putative | QDO/A (1) | ||
| PF3D7_1029900 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1032000 | ribosome maturation factor RimM, putative (RimM) | TDO/A (1) | ||
| PF3D7_1103100 | 60S acidic ribosomal protein P1, putative (RPP1) | TDO/A (1) | ||
| PF3D7_1105100 | histone H2B (H2B) | TDO/A (2) | ||
| PF3D7_1107300 | polyadenylate-binding protein-interacting protein 1, putative (PAIP1) | TDO/A (1) | ||
| PF3D7_1108600 | endoplasmic reticulum-resident calcium binding protein (ERC) | TDO/A (1) | KVYF | |
| PF3D7_1116200.1 | pyridoxine biosynthesis protein PDX2 (PDX2) | QDO/A (1) | ||
| PF3D7_1117700 | GTP-binding nuclear protein ran/tc4 (RAN) | QDO/A (1) | RQIQF | |
| PF3D7_1121600 | circumsporozoite-related antigen,exported protein 1 (EXP1) | TDO/A (1) | ||
| PF3D7_1127900 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1141400 | phosphatidylinositol N-acetylglucosaminyltransferase subunit H, putative (PIGH) | TDO/A (1) | ||
| PF3D7_1149000 | antigen 332, DBL-like protein (Pf332) | TDO/A (1) | KIIW | |
| PF3D7_1206800 | conserved Plasmodium protein, unknown function | QDO/A (1) | ||
| PF3D7_1210600 | conserved Plasmodium protein, unknown function | QDO/A (1) | ||
| PF3D7_1211200 | conserved Plasmodium protein, unknown function | TDO/A (1) | KVKF/KRINF/KYIEF | |
| PF3D7_1214100 | GPI ethanolamine phosphate transferase 3, putative (PIGO) | TDO/A (1) | ||
| PF3D7_1222300 | endoplasmin, putative (GRP94) | TDO/A (1) | ||
| PF3D7_1224000 | GTP cyclohydrolase I (GCH1) | TDO/A (1) | ||
| PF3D7_1227000 | conserved Plasmodium protein, unknown function | TDO/A (2) | ||
| PF3D7_1227700 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1228600 | merozoite surface protein 9 (MSP9) | QDO/A (1) | ||
| TDO/A (1) | ||||
| PF3D7_1229400 | macrophage migration inhibitory factor (MIF) | QDO/A (1) | ||
| TDO/A (1) | ||||
| PF3D7_1231600 | pre-mRNA-splicing factor ATP-dependent RNA helicase PRP2, putative (PRP2) | TDO/A (1) | ||
| PF3D7_1234600 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1234900 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1240600 | erythrocyte membrane protein 1, PfEMP1 (VAR) | TDO/A (1) | ||
| PF3D7_1249800 | THO complex subunit 2, putative (THO2) | TDO/A (1) | ||
| PF3D7_1303800 | conserved Plasmodium protein, unknown function | TDO/A (2) | ||
| PF3D7_1306400 | 26S protease regulatory subunit 10B, putative (RPT4) | TDO/A (1) | ||
| PF3D7_1308400 | conserved Plasmodium protein, unknown function | QDO/A (1) | RRVLF | |
| PF3D7_1308800 | tyrosine recombinase (INT) | QDO/A (1) | KYIKF | |
| TDO/A (1) | ||||
| PF3D7_1316100 | inositol polyphosphate kinase, putative (IPK2) | TDO/A (1) | ||
| PF3D7_1324800 | dihydrofolate synthase/folylpolyglutamate synthase (DHFS-FPGS) | QDO/A (1) | ||
| PF3D7_1326600 | conserved Plasmodium protein, unknown function | QDO/A (2) | ||
| PF3D7_1336600 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1342300 | tetratricopeptide repeat family protein, putative | TDO/A (1) | ||
| PF3D7_1342900 | transcription factor with AP2 domain(s) (ApiAP2) | TDO/A (1) | ||
| PF3D7_1346400 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1348400 | conserved Plasmodium membrane protein, unknown function | TDO/A (1) | ||
| PF3D7_1351200 | conserved Plasmodium protein, unknown function | TDO/A (1) | KYVNW | |
| PF3D7_1356900 | protein kinase 5 (PK5) | TDO/A (1) | ||
| PF3D7_1357400 | conserved Plasmodium protein, unknown function | QDO/A (1) | KSIRF | |
| PF3D7_1360700 | E3 SUMO-protein ligase PIAS, putative (PIAS) | QDO/A (1) | KKVLW | |
| PF3D7_1361200 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1366400 | rhoptry protein (Rhop148) | TDO/A (1) | KILF | |
| PF3D7_1417600 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1423700 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1444300 | 1-acyl-sn-glycerol-3-phosphate acyltransferase, putative (LPAAT) | TDO/A (1) | ||
| PF3D7_1456500 | conserved Plasmodium protein, unknown function | QDO/A (2) | ||
| PF3D7_1457000 | signal peptide peptidase (SPP) | QDO/A (1) | ||
| PF3D7_1460500 | conserved Plasmodium protein, unknown function | TDO/A (1) | KVDF/KTVSF | |
| PF3D7_1460700 | 60S ribosomal protein L27 (RPL27) | TDO/A (1) | ||
| PF3D7_1464500 | conserved Plasmodium membrane protein, unknown function | TDO/A (1) | ||
| PF3D7_1465900 | 40S ribosomal protein S3, putative | TDO/A (1) | ||
| PF3D7_1466300 | 26S proteasome regulatory subunit RPN2, putative (RPN2) | TDO/A (1) | ||
| PF3D7_1470800 | conserved Plasmodium protein, unknown function | TDO/A (1) | ||
| PF3D7_1471900 | conserved Plasmodium protein, unknown function | TDO/A (1) | KIIEF |
aAnnotations available on PlasmoDB database (v24)
bSelection medium used for the growth of isolated yeast clones were indicated. Numbers indicate the count of isolated clones. TDO/A Triple dropout supplemented with Aureobasidin A, QDO/A Quadruple dropout supplemented with Aureobasidin A
cThe presence and the sequence of RVxF binding motifs were indicated
dShared proteins isolated by affinity purification and Y2H screening