Fig. 4.

Recovery of protein synthesis in roots after removal of plants from MG132. Plants were treated with or without MG132 for 4 h, after which plants were washed and placed into Hoagland’s media without MG132 for 0.5 and 3 h. a Immunoreactive proteins containing puromycin were detected on a 15 % SDS-PAGE gel. Smaller letters in parenthesis represent band intensity relative to control on dried membranes using image-J. Images are representative of two other experimental replicates. Membranes were stained with Ponceau Red prior to immunblotting. b Immunoblot of polyubiquitinated proteins and Cox2