Erratum to: Development of a Multiplex-PCR probe system for the proper identification of Klebsiella variicola

Erratum

The original version of this article [1] unfortunately contained a mistake in Table 1. The primer sequence which corresponds to KmtnC-R in the table 1 was provided incorrectly. The correct nucleotide sequence is:

KmtnC-R:GCATGGCCCAGGTGTTCAG

An updated version of Table 1 has been provided below.

Table 1.

Amplification conditions, oligonucleotide combinations, sequence and amplification fragment of multiplex-PCR for K. variicola identification

Amplification conditionsa	Name of combination primers	Shared unique genes, oligonucleotides and sequence (5′- 3′) of each bacterial specie
		K. pneumoniae	Amplification fragment (bp)	K. variicola	Amplification fragment (bp)	Klebsiella spp.	Amplification fragment (bp)
		phosphohydrolase		phosphoglycerate mutase		phosphopentane phosphatase (mtnC)
1	M-PCR-1	KP888-F: AAGCAAGCCAGAACAGAAAG	888	KV770-F: TCCCGAGGTTCACATTTCC	449	KmtnC-F: CCGCCGACCTTATCACTAC	340
		KP888-R: ACTTCGGTTTTATCCAGGTC		KV770-R: AGCGGGTGAACGTCGATAC		KmtnC-R: GCATGGCCCAGGTGTTCAG
		transferase (yphG)		N-acetyltransferase		phosphopentane phosphatase (mtnC)
1	M-PCR-2	KP878-F: ACCGATAACCAGCCTGACTT	878	KV1615-F: ACACAACATTTCAGGCGGCT	499	KmtnC-F: CCGCCGACCTTATCACTAC	340
		KP878-R: CTTTCTTCTGCCCACTGTTG		KV1615-R: GGGCGTGGCTTTTTTCATCG		KmtnC-R: GCATGGCCCAGGTGTTCAG
		phosphohydrolase		thiopurine S-methyltransferase		phosphopentane phosphatase (mtnC)
2	M-PCR-3	KP888-F: AAGCAAGCCAGA ACAGAAAG	888	KV1000-F: CTGGGATGTGGCAATGGTG	438	KmtnC-F: CCGCCGACCTTATCACTAC	340
		KP888-R: ACTTCGGTTTTATCCAGGTC		KV1000-F: AAACTGCGCCTGCTGTATC		KmtnC-R: GCATGGCCCAGGTGTTCAG

^aMultiplex-PCR conditions used under the oligonucleotides combinations. 1: 5pmol/reaction of K. variicola and Klebsiella spp, 25pmol/reaction of K. pneumoniae; 2: 25 pmol/reaction of K. pneumoniae, 5 pmol/reaction of K. variicola and 1 pmol/reaction of Klebsiella spp