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. Author manuscript; available in PMC: 2016 Mar 17.
Published in final edited form as: Nat Protoc. 2011 Mar 18;6(4):457–467. doi: 10.1038/nprot.2010.202

Figure 1.

Figure 1

Schematic diagrams of xMD and LCM. (a) Schematic showing the principle of xMD. Target cells are selectively immunolabeled, covered with an EVA film and the entire tissue section is irradiated. The transient temperature increases focally at the site at which the chromogen melts the EVA polymer and bonds it to underlying target cells. No microscopic visualization or operator-based cell selection is required during the xMD process. (b) Schematic showing the principle of standard laser capture microdissection. First, under direct microscopic visualization, the operator identifies target cells in the tissue section. Then, the laser is manually fired at the targets in a one-by-one manner to generate a heat transient that melts EVA and bonds it to the underlying cells.