Table 5. Various Reports of ESC Differentiation to Hepatocytes.

This table summarizes the various reports on the generation of ESC-derived hepatocytes from different species’ cells. Table includes the reference, species of cell used and a brief summary of the growth factors, cytokines and culture techniques used within each reference’s differentiation protocol. The forward slashes in the “summary of protocol” column denote a change in culture conditions consisting of a basal medium or growth factor change. This table is not an exhaustive list and is meant to be representative.

Reference	Species	Summary of Protocol
(Agarwal et al., 2008)	Human	ActA/FGF4, HGF/BSA, FGF4, HGF/FGF4, HGF, OSM, DEX
(Basma et al., 2009)	Human	EB formation/ActA, FGF2/HGF, DMSO/DEX
(Bukong et al., 2012)	Human	ActA, FGF2/FGF4, BMP2, LY294002/FGF10, RA, FGF4, HGF, EGF, SB431542/FGF10, RA, FGF4, HGF, EGF, SB431542, OSM, DEX
(Cai et al., 2007)	Human	ActA/FGF4, BMP2/HGF/OSM, DEX
(Duan et al., 2010)	Human	ActA/FGF4, HGF, BMP2, BMP4/FGF4, HGF, BMP2, BMP4, DMSO/FGF4, HGF, OSM, DEX, DMSO
(Hay et al., 2008b)	Human	ActA, NaBut/ActA, NaBut/DMSO/HGF, OSM
(Medine et al., 2011)	Human	ActA, Wnt3a/DMSO/HGF, OSM
(Sharma et al., 2009)	Human	DMSO/NaBut/SNAP
(Touboul et al., 2010)	Human	ActA, FGF2/LY294002, ActA, BMP4, FGF2/FGF10/FGF10, RA, SB431542/FGF4, HGF, EGF
(Tuleuova et al., 2010)	Mouse	Use of various growth factor arrays and micropatterned co-cultures to induce hepatic differentiation
(Farzaneh et al., 2014)	Human	Serum- and xenobiotic-free, simple, and cost-effective ECM, “RoGel,” developed
(Szkolnicka et al., 2014)	Human	Wnt3a, ActA/KO-serum, DMSO/HGF, OSM
(Sengupta et al., 2014)	Human	Albumin fraction V, ActA/FGF4, BMP2/HGF, OSM, DEX