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. Author manuscript; available in PMC: 2017 Mar 14.
Published in final edited form as: Cancer Cell. 2016 Mar 14;29(3):367–378. doi: 10.1016/j.ccell.2016.02.012

Figure 6.

Figure 6

Effect of the PKCι-ELF3-NOTCH3 axis on asymmetric cell division and tumor initiation. (A) Immunofluorescence of A549 oncosphere cells for CD133 (red) and DAPI (blue) during interphase (a–c) and mitosis (d–i) demonstrating symmetric (d–f) and asymmetric (g–i) cell divisions. Scale bars 10 μm. (B) PKCι, ELF3 and NOTCH3 KD inhibit mitotic index in LADC oncosphere cells. Data expressed as mitotic index (% mitotic cells) +/−SEM. n=3. *p<0.05 compared to NT. (C) Effect of PKCι, ELF3 and NOTCH3 KD on asymmetric cell division. Results expressed as % asymmetric cell divisions (>300 mitoses evaluated/cell line). *p≤0.0001 compared to NT using Fisher exact test. (D) Bioluminescent images of representative tumor bearing-mice 5 weeks after injection of 50,000 NT, PKCι KD, ELF3 KD or NOTCH3 KD A549 oncosphere cells. (E) Tumor size expressed as bioluminescence flux +/−SEM, n=10. *p<0.05 compared to NT control. (F) Representative bioluminescence images of lung tumors ex vivo. (G) Kaplan-Meier survival analysis of mice injected with the indicated A549 oncosphere cells (50,000 cells). N=10. *p<0.05 compared to NT. (H) QPCR for ELF3, NOTCH3 and CD133. Results are expressed as fold parental +/−SEM, n=10, *p<0.05 compared to NT parental, **p<0.05 compared to injected NT oncosphere cells.

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